Lambda Light Chain Antibody / Lambda Antibody orb385740 from Biorbyt

Description

This mAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant

Product Specs
ItemLambda Light Chain Antibody / Lambda Antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb385740
Quantity20 µg, 100 µg
ApplicationsFACS, IF, IHC-P, WB
Conjugate/TagUnconjugated
PurityProtein G Affinity Chromatography
ReactivityHuman
TargetLambda Light Chain
IsotypeMouse IgG2a, kappa
CloneHP6054
HostMouse
ImmunogenPurified human IgG myeloma proteins were used as the immunogen for this Lambda Light Chain antibody
ClonalityMonoclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the Lambda Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide)

Specifications/Features

Application Notes: The optimal dilution of the Lambda Light Chain antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Dilutions: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

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